JBC members are provided access to expertise to successfully perform CyTOF single-cell phenotyping of cells from patients or mice.  Cytof core services include consultation on experimental design, cell preparation and staining methods, reagents for cytof-ready antibody collections for both human and mouse markers as well as data analysis which include dimensional reduction analysis and statistics via the JBC Cytobank Account

Mechanism

Flow Cytometry

  • Fluorescent-tagged antibodies or binding reagents
  • Limited marker analysis due to spectral overlap

 

CyTOF

  • Antibodies tagged with rare earth metal isotopes
  • Minimal spectral overlap
  • Low background in cells