JBC members are offered a highly innovative method for the generation, manipulation and in vivo testing of murine myeloid lineages using HoxB8 cell manipulation.

Mechanism:

Hoxb8 is a homeobox gene that inhibits myeloid differentiation. Integration of a murine stem cell virus encoding HoxB8 under the control of mutant estrogen receptor promotes myeloid progenitor proliferation in the presence of supraphysiologic levels of β- estradiol and stem cell factor (SCF). HoxB8 progenitors exhibit the phenotype of common myeloid progenitors. Upon β-estradiol withdrawal, HoxB8 cells differentiate into genetically normal daughter cells.

HOXB8 MECHANISM

  • HoxB8 progenitors are generated easily from any strain or background, expand indefinitely, and can be transported easily.
  • HoxB8 technology is centralizable.
  • HoxB8 progenitors can differentiate into a large range of daughter lineages relevant for arthritis by varying the growth factor profile employed during culture.
  • HoxB8 progenitors are easily transducible using viral vectors, allowing CRISPR or shRNA targeting of anygene of interest
  • HoxB8 daughter cells can be engrafted into live mice.

HOXB8 CELL DIFFERENTIATION UPON β-ESTRADIOL WITHDRAWAL